@InProceedings{GregóriodaSilvaCarvFerr:2015:DeLeIn,
author = "Greg{\'o}rio da Silva, Bruno C{\'e}sar and Carvalho-Tavares,
Juliana and Ferrari, Ricardo Jos{\'e}",
affiliation = "{Federal University of S{\~a}o Carlos} and {Federal University of
Minas Gerais} and {Federal University of S{\~a}o Carlos}",
title = "Detection of leukocytes in intravital video microscopy based on
the analysis of Hessian matrix eigenvalues",
booktitle = "Proceedings...",
year = "2015",
editor = "Papa, Jo{\~a}o Paulo and Sander, Pedro Vieira and Marroquim,
Ricardo Guerra and Farrell, Ryan",
organization = "Conference on Graphics, Patterns and Images, 28. (SIBGRAPI)",
publisher = "IEEE Computer Society",
address = "Los Alamitos",
keywords = "detection of leukocytes, intravital microscopy, temporal image
registration.",
abstract = "Detection of rolling and adhered leukocytes in intravital
microscopy image sequences is an important task in studies of
leukocyte-endothelial interactions in the microcirculation of
living small animals under different inflammatory conditions. This
procedure is usually performed by visual assessment of the image
sequences. However, despite being tedious and time consuming, this
procedure is prone to the inter- and intra-observer variability.
In this work, we developed an automated computer system for the
detection of leukocytes in intravital video microscopy. First, the
video frames were processed by the bilateral filter to reduce
noise while preserving sharp edges. Then, a demons-based image
registration technique was applied to minimize animal motion.
Finally, the detection of leukocytes was performed by local
analysis of Hessian matrix eigenvalues. Quantitative and
qualitative evaluation of the proposed method were conducted by
using 220 video frames obtained from an experimental study
performed on the brain microvasculature of mice. The proposed
method was compared with the template matching technique using
precision, recall and F1-Score measures. For the Hessian-based
method, the results of precision, recall and F1-Score were,
respectively, equal to 0.81, 0.86, and 0.83. For direct
comparison, the results obtained for the template matching
technique were 0.86, 0.73 and 0.79.",
conference-location = "Salvador, BA, Brazil",
conference-year = "26-29 Aug. 2015",
doi = "10.1109/SIBGRAPI.2015.48",
url = "http://dx.doi.org/10.1109/SIBGRAPI.2015.48",
language = "en",
ibi = "8JMKD3MGPBW34M/3JMNT98",
url = "http://urlib.net/ibi/8JMKD3MGPBW34M/3JMNT98",
targetfile = "manuscript_sibigrapi_2015.pdf",
urlaccessdate = "2024, Apr. 30"
}